In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine
Identifieur interne : 001761 ( Main/Exploration ); précédent : 001760; suivant : 001762In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine
Auteurs : M. Lusakibanza [République du Congo] ; G. Mesia [République du Congo] ; G. Tona [République du Congo] ; S. Karemere [République du Congo] ; A. Lukuka [République du Congo] ; M. Tits [Belgique] ; L. Angenot [Belgique] ; M. Frederich [Belgique]Source :
- Journal of ethnopharmacology [ 0378-8741 ] ; 2010.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Plante médicinale, Homme.
English descriptors
- KwdEn :
Abstract
Aim of the study: The in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ethnobotanical survey conducted in D.R. Congo and focusing on plants used traditionally to treat malaria. The in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by Plasmodium berghei berghei. Materials and methods: The growth inhibition of Plasmodiumfalciparum strains was evaluated using the measurement of lactate dehydrogenase activity. The extracts (aqueous, CH3OH, EtOH and CH2Cl2) were prepared by maceration and tested in vitro against the 3D7 (chloroquine sensitive) and W2 (chloroquine resistant) strains of Plasmodiumfalciparum and against the human normal fetal lung fibroblasts WI-38 to determine the selectivity index. Some extracts were also used at the dose of 300 mg/kg to evaluate their activity in mice infected since 4 days by Plasmodium berghei. Results: Two plants presented a very high activity (IC50 < 3 μg/ml). These plants were Strychnos icaja roots bark (MeOH and CH2Cl2) and Physalis angulata leaves (MeOH and CH2Cl2). One plant (Anisopappus chinensis whole plant, MeOH and CH2Cl2) presented a high activity (IC50 < 15 μg/ml). The extracts of Anisopappus chinensis and Physalis angulata showed also a good inhibition of parasitemia in vivo. Flavonoids, phenolic acids and terpenes were identified in these plants by a general phytochemical screening method. Conclusion: Three plants showed a very interesting antiplasmodial activity (Anisopappus chinensis, Physalis angulata and Strychnos icaja) and one of them showed a good selectivity index (>10, Anisopappus chinensis). Anisopappus chinensis and Physalis angulata were also active in vivo.
Affiliations:
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<series><title level="j" type="main">Journal of ethnopharmacology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antimalarial</term>
<term>Cytotoxicity</term>
<term>Fibroblast</term>
<term>Folk medicine</term>
<term>Human</term>
<term>In vitro</term>
<term>Loganiaceae</term>
<term>Medicinal plant</term>
<term>Meliaceae</term>
<term>Parasiticide</term>
<term>Pesticide crop</term>
<term>Pharmacognosy</term>
<term>Plant origin</term>
<term>Solanaceae</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>In vitro</term>
<term>Antipaludique</term>
<term>Antiparasitaire</term>
<term>Cytotoxicité</term>
<term>Plante médicinale</term>
<term>Médecine traditionnelle</term>
<term>Homme</term>
<term>Fibroblaste</term>
<term>Pharmacognosie</term>
<term>Origine végétale</term>
<term>Meliaceae</term>
<term>Solanaceae</term>
<term>Plante pesticide</term>
<term>Loganiaceae</term>
<term>Entandrophragma</term>
<term>Melia azedarach</term>
<term>Physalis angulata</term>
<term>Strychnos</term>
<term>Plante pharmaceutique</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante médicinale</term>
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<front><div type="abstract" xml:lang="en">Aim of the study: The in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ethnobotanical survey conducted in D.R. Congo and focusing on plants used traditionally to treat malaria. The in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by Plasmodium berghei berghei. Materials and methods: The growth inhibition of Plasmodiumfalciparum strains was evaluated using the measurement of lactate dehydrogenase activity. The extracts (aqueous, CH<sub>3</sub>
OH, EtOH and CH<sub>2</sub>
Cl<sub>2</sub>
) were prepared by maceration and tested in vitro against the 3D7 (chloroquine sensitive) and W2 (chloroquine resistant) strains of Plasmodiumfalciparum and against the human normal fetal lung fibroblasts WI-38 to determine the selectivity index. Some extracts were also used at the dose of 300 mg/kg to evaluate their activity in mice infected since 4 days by Plasmodium berghei. Results: Two plants presented a very high activity (IC<sub>50</sub>
< 3 μg/ml). These plants were Strychnos icaja roots bark (MeOH and CH<sub>2</sub>
Cl<sub>2</sub>
) and Physalis angulata leaves (MeOH and CH<sub>2</sub>
Cl<sub>2</sub>
). One plant (Anisopappus chinensis whole plant, MeOH and CH<sub>2</sub>
Cl<sub>2</sub>
) presented a high activity (IC50 < 15 μg/ml). The extracts of Anisopappus chinensis and Physalis angulata showed also a good inhibition of parasitemia in vivo. Flavonoids, phenolic acids and terpenes were identified in these plants by a general phytochemical screening method. Conclusion: Three plants showed a very interesting antiplasmodial activity (Anisopappus chinensis, Physalis angulata and Strychnos icaja) and one of them showed a good selectivity index (>10, Anisopappus chinensis). Anisopappus chinensis and Physalis angulata were also active in vivo.</div>
</front>
</TEI>
<affiliations><list><country><li>Belgique</li>
<li>République du Congo</li>
</country>
<region><li>Province de Liège</li>
</region>
<settlement><li>Liège</li>
</settlement>
<orgName><li>Université de Liège</li>
</orgName>
</list>
<tree><country name="République du Congo"><noRegion><name sortKey="Lusakibanza, M" sort="Lusakibanza, M" uniqKey="Lusakibanza M" first="M." last="Lusakibanza">M. Lusakibanza</name>
</noRegion>
<name sortKey="Karemere, S" sort="Karemere, S" uniqKey="Karemere S" first="S." last="Karemere">S. Karemere</name>
<name sortKey="Lukuka, A" sort="Lukuka, A" uniqKey="Lukuka A" first="A." last="Lukuka">A. Lukuka</name>
<name sortKey="Mesia, G" sort="Mesia, G" uniqKey="Mesia G" first="G." last="Mesia">G. Mesia</name>
<name sortKey="Tona, G" sort="Tona, G" uniqKey="Tona G" first="G." last="Tona">G. Tona</name>
</country>
<country name="Belgique"><region name="Province de Liège"><name sortKey="Tits, M" sort="Tits, M" uniqKey="Tits M" first="M." last="Tits">M. Tits</name>
</region>
<name sortKey="Angenot, L" sort="Angenot, L" uniqKey="Angenot L" first="L." last="Angenot">L. Angenot</name>
<name sortKey="Frederich, M" sort="Frederich, M" uniqKey="Frederich M" first="M." last="Frederich">M. Frederich</name>
</country>
</tree>
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</record>
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